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급성췌장염으로 인한 혈장 및 간장의 지질변동에 관한 실험적 연구

급성췌장염으로 인한 혈장 및 간장의 지질변동에 관한 실험적 연구
Other Titles
Experimental studies on the changes of lipids in plasma and liver tissue in acute pancreatitis
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연세대학교 대학원
[영문] The association of plasma lactescence with pancreatitis in man was first observed approximately 100 years ago. Since that time, there has been various clinical and experimental reports concerning the appearance of hyperlipemia associated with pancreatitis. However, the mechanism by which pancreatitis is associated with the alteration of plasma lipids is obscure. The present experiment is undertaken to clearify the relationship of total plasma lipids, lipid phosphorus, cholesterol and serum lipase in experimental pancreatitis and also to observe the lipid change in liver tissue. The rabbits weighing approximately 2kg irrespective of sect, were used and divided into the following groups: 1) Intraductal instillation of 1% sodium desoxycholate in saline and complete ligation of the pancreatic duct; 2) Intraductal instillation of 20% ethyl alcohol in saline and complete ligation of the pancreatic duct; 3) Intraductal instillation of 10% bile in saline and complete ligation of the pancreatic duct; 4) Intraductal instillation of saline and complete ligation of the pancreatic duct as a control; 5) Simple ligation of the pancreatic duct as a control. The abdomen of the rabbit was opened through an upper midline incision under sodium pentothal anesthesia and aseptic technique was used throughout the operative procedure. The major pancreatic duct, which enters into the duodenum at a point approximately 20cm from the pylorus, was easily cannulated in its intramural portion with a small polyethylene tube. With a syringe 2.0ml of each prepared solution was infused slowly without too much pressure. Then, the cannula was removed during libation of the pancreatic duct, and the abdominal incision was closed. The blood samples were drawn 12, 24, 36, 48, 72 hours, one week, two weeks, three weeks following the surgery, these samples were studied for serum lipase activity, total lipid, lipid phosphorus, cholesterol. When the animal died or wart sacrificed, the pancreatic tissue and liver tissue were fixed in 10% formalin, and routine hematoxylin-eosin stains were done after sectioning the paraffin embedded tissue. Some of the liver tissue was prepared as a fat-staining with "Oil-red-0". In order to determine total lipid in liver tissue, five grams of liver tissue was weighed on a torsion balance immediately following sacrifice of animal, dried in an oven with temperature ranging 60∼70 degree centigrade for about twelve hours. This dessicated liver tissue was extracted with chloroform and transfered to a pre-measured flask, and the chloroform was evaporated in a hot water bath at 50 degree centigrade. Then, the specimen wart kept in a dessicater for at least six hours, following which it's weight was determined on the chemical balance. The serum lipase was determined by the Cherry and Grandall's Method; total lipid by modified Sperry and Brand's Method: lipid phosphorus by the modified Youngburg's Method: cholesterol by the Kingsley's modified Yanasawa Method. Results Animal died or sacrificed within one week fellowing surgery, showed focal or diffuse parenchymal necrosis, and intra-pancreatic and peri-pancreatic fat necrosis typical of acute hemorrhagic pancreatitis, and those who survived until the third post-operative week showed findings of chronic pancrestitis namely atrophy of pancreatic lobules, ductal transformation of acini, dilation of the pancreatic duct, inter and intra-lobular fibrosis and chronic inflammatory cellular infiltration. The histological examination of liver tissue revealed no remarkable fat metamorphosis. The maximum rise in serum lipase usually occurred on the first post-operative day and returned to pre-operative levels on the third day. Thereafter, the lipase level was stabilized and did net vary significantly throughout the duration of the experiment. The elevation of the lipase activity was noted in saline control group although the simple ligation group showed a remarkably lower lipase level than the study group. The maximum rise in plasma total lipid, lipid phosphorus, cholesterol usually occurred on the second to the third day following the instillation of various solution, declined about one week following the operation, then, gradually returned to the pre-operative level by the third week. The experimental groups showed remarkably higher value than the control groups. Total lipid content of liver tissue in the study groups was not remarkably higher than the control groups. However, the total lipid level of liver tissue was parallel with the plasma total lipid level.
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2. 학위논문 > 1. College of Medicine (의과대학) > 박사
Yonsei Authors
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