인삼이 Adrenaline 투여로서 발생된 각장기의 변화에 미치는 영향에 관한 실험적 연구
Experimental studies on the effect of Ginseng on the changes of various organs produced by repeated adrenaline injection in the rabbits
Early in 1903, Josue called attention on the resemblance of human
arteriosclerosis to the lesions he produced in the rabbits by repeated injection of
adrenaline through the ear veins. Since that time this method has repeatedly been
proven to be one of the most reliable methods of producing aortic lesions
experimentally. However, it was later learned that the lesions so produced actually
are in the aortic media rather than in the intima which is the usual form of
atherosclerosis or arteriosclerosis.
Further investigations proved that other substances such as diphtheric toxin,
tyramine and other vasotonic subsatnces can also produce similar medial changes as
in adrenaline injection. Interests in this field continued since the medial changes
were very similar to socalled idiopathic cystic medial necrosis seen in human arota
which are often associated with dissecting aneurysm.
The interest in the effect of Ginseng continues to attract many investigators
since in spite of its advocated curious effects claimed by many people in the
orient, there have been very little scientific confirmation of its value to such an
Knowing the backgrounds associated with the problem of Ginseng, it was the
purpose of this investigator to see the influence of Ginseng on the changes
produced by adrenaline injection by means of pathological evaluation.
Materials and Methods:
Healthy Korean albino rabbits weighing around 2.0Kg. were used. The animals were
divided into 5 groups and were treated as follows:
Group 1.: 6 animals receiving daily injection of saline into the ear veins for 8
weeks in addition to regular diet. This constituted the control group.
Group 2.: 6 animals receiving daily injection of adrenaline into the ear veins
for 8 weeks. 0.1 c.c. during the first week and 0.2c.c. during the remainder.
Group 3.: 6 animals receiving 2.0gm of Ginseng powder mixed with the diet and
daily saline injection for 4 weeks and daily adrenaline injection for 4 weeks
Group 4.: 6 animals receiving Ginseng powder as well as adrdenaline injection
daily for 8 weeks.
Group 5.: 6 animals receiving daily adrenaline injection for 4 weeks and Ginseng
administration for 4 weeks thereafter.
After completion of above experiment all animals were sacrificed and examined
grossly. After adequate examination the organs were immediately fixed into 10%
neutral formalin and embedded into paraffin. Histological sections were stained
with hematoxylin and eosin routinely. Sections from various portions of aorta and
heart were stained with phosphotungstic acid hematoxylin, Periordic Acid Schiff
reaction, Elastic stain in addition to H. & E.
Results and conclusion:
The control animals did not show any abnormalities as expected. The adrenaline
injection produced profound and devastating damage to the aortic media which were
more pronounced in the ascending and thoracic aorta than in the abdominal aorta.
The degeneration as it appears to seems affect the muscle fibers earlier and more
severely than the other elements of the aorta. Atrophy and loss of muscle fibers
created separation of elastic membranes causing cystic changes and later produced
strikingly regular but diffuse dissolution of media. Sometimes of calcium salts was
The animals receiving Ginseng powder with the adrenaline injection also showed
some degenerative effects in the media, however, it s extent and severity were far
less than seen in the animals receiving adrenaline alone. Adrenaline injection also
caused sever panarteritis in the arterioles of myocardium with resultant myocardial
damages. None of the animals receiving Ginseneg showed this phenomenon. Arteriolar
thickening was also observed int e kidneys in the adrenaline injected animals but
not in the other animals. Other minor changes are also described and compared as to
their nature and severity.
The results obtained in the present investigation strongly suggests that the
Ginseng acts favorably for the cure and prevention of diffuse medial degeneration
of aorta and other vascular changes in various organs produced by adrenaline
injection. It is regrettable that the exact mechanism(s) for such effect can not be
adequately presented at this time. Perhaps reported effects of Ginseng in lowering
blood pressure might have some connection with such a beneficial effect though it
is purely speculative at this time. Further investigation certainly is wanted.