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Prednisolone의 경구투여가 결핵반응에 미치는 영향에 관한 실험적 연구

Other Titles
 (The) effect of prednisolone on tuberculin reaction in guinea pigs 
Authors
 김덕희 
Issue Date
1972
Description
의학과/석사
Abstract
[한글]
[영문] Stoerk(1950) reported that the administration of cortisone or ACTH to guinea pigs, vaccinated with BCG, will attenuate or block the tuberculin reaction. Derbes et al(1950) and Cummings et al(1952) confirmed this. Tuberculin skin reaction is the prototype of delayed hypersensitivity and contact dermatitis, homograft rejection phenomena and autoimmune disease are a kind of delayed type of hypersensitivity. The present studies are undertaken in an attempt to identify the mechanism of effects of prednisolone on tuberculin reaction. Material and Method Forty-five albino guinea pigs, each weighting 400 to 600 gm, were injected with 1cc(20mg/cc) of BCG intraperitoneally. After 3 weeks, a PPD skin test was done and the animals which showed a positive reaction were for the experiment and divided into the following three groups: 1) Control group 2) Predisolone treated group was subdivided into those having doses of 1mg/kg and 4mg/kg. Before administration of prednisolone, WBC, WBC differential count and antibody titer to tuberculosis were checked and compared with the results after administration of the drug. The prednisolone was administered per oral route, daily, for 8 weeks. PPD skin test was done once every week and the size of induration was read 48 hours after the test done. When the tuberculin reaction was markedly suppressed with prednisolone, passive transfer of tuberculin hypersensitivity with viable leukocytes was tried and a histological examination was done with routine hematoxylin-Eosin staining technique. Result and Discussion The Decreased tuberculin skin reaction occurred 1 week after administration and the marked inhibition of tuberculin skin reaction occurred 5 weeks after administration. The group treated with prednisolone, dosage of 4mg/kg, was much more inhibited than the group having doses of 1mg/kg for 4 weeks. From 5 weeks after administration of prednisolone, there was no difference between the 2 groups. The recovery from tuberculin skin reaction occurred within 48 hours after discontinuing the administration of prednisolone. When the tuberculin skin reaction was markedly suppressed, antibodies to tuberculosis were not decreased accordingly and the leukocytes derived from the guinea pigs could transfer immunity to normal guinea pigs, even though with depressed skin reaction. Lymphopenia and eosinopenia occurred after the prednisolone administration and histologic examination of the skin tested showed a marked decrease of lymphocytes in the area. Regarding the mechanism of action on the tuberculin reaction, Germuth et al(1952) stated that the same mechanism was involved in the suppression of nonspecific inflammatory reactions. Leathy & Morgan(1952) reported that cytotoxic action of PPD was inhibited by pretreatment of prednisolone. Wasserman(1967) also reported prednisolone inhibited the synthosis of DNA and RNA, and suppressed the mitosis of lymphocytes occurring after contact with antigen. The mechanism of prednisolone in inhibiting tuberculin reaction is still unknown and requires further study.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/115261
Appears in Collections:
2. 학위논문 > 1. College of Medicine (의과대학) > 석사
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