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전류가 골절치유에 미치는 영향에 대한 실험적 연구

Title
 전류가 골절치유에 미치는 영향에 대한 실험적 연구 
Other Titles
 (The) effect of electric current on fracture healing 
Issue Date
1974
Publisher
 연세대학교 대학원 
Description
의학과/박사
Abstract
[한글]
[영문] Since the first indication of a piezoelectric effect in biological tissue was reported by Martin (1941), several investigators have been concerned in many experimental studies with its effect on bone formation. By measuring the electrical potentials of the compressed bone, Yasuda et at. (1955) offered a suggestion that dynamic energy could be transformed into electric energy in the formation of callus. With the succeeding researches relating to the effect of electrical potentials on ossification or repair of fracture, the mechanism and processes have been widely postulated and also demonstrated(Becker, 1961; Bassett and Becker, 1962: Bassett and Pauluck, 1964; 0'Coner, 1969: Friedenberg et al., 1970, 1971). In order to elucidate the possible problems arising from the application of electric current in the management of fractures, this investigation was designed as follows. Sixty four(about 2.0 kg) albino rabbits (128 fibulae) were used in this experimental study. The animals were divided into 4 groups of rabbits; thus each group contained 16 rabbits. The animals were operated in sterile condition under nembutal anesthesia (30mg per kg of body weight). An antero-lateral skin incision was made over the knee joint and the fibula was exposed and a transverse fracture was made at the neck of the fibula using a rotating dental disc. We drilled a 0.2cm holes thru the fibula cortex 5mm proximal, distal, or both to the fracture site and at the fracture site itself using a Kirschner wire for the left side according to the experimental groups to be described. Then the electrical implants, which consisted of a mercury cell and 150k resistors designed to deliver a constant 4-6μa were inserted into the thigh subcutaneously. We performed an identical surgical procedure on the right side as on the left except that the leads were not connected to a cell, for the control study. In group Ⅰ a cathode was inserted 5mm proximal and an anode 5mm distal to the fracture site. In group Ⅱ a anode was inserted into the fracture site and an anode was inserted 5mm proximal to the fracture site. In group Ⅲ an anode was inserted into the fracture site and a cathode was inserted 5mm proximal to the fracture site. In group Ⅳ a cathode was inserted into the fracture sits and an anode was anchored intramuscularly 5mm proximal to the fracture site. The operated animals were sacrificed with intervals of 3 days, 5 days, and 1, 2, 3, 4, 5, and 7 weeks respectively after fracture. In each case 24 hours prior to sacrifice 0.33mCi per kg of body weight of Ca**45 (CaCl^^2 in 0.5mHCl with a specific activity 12.2mCi/mg) was injected by the intravenous route. After the sacrifice, the entire fibula attached to the tibia was resected, X-rayed, and placed in 10% neutral formalin solution and fixed for 24 hours. After fixation it was decalcified and paraffineblocks were made. Multiple sections of each specimen which were cut at five micra, were coated with Kodak NTB-3 nuclear emulsion and allowed to expose for periods of twenty one days. The autoradiographs were developed with Kodak D-19 developer and fixed with Kodak fixer and stained with hematoxylin and eosin. The autoradiographic technique was based on the method of Messier and Leblond. The results observed in this study were mainly concentrated on the gross findings of the fracture. radiological findings, microscopic findings, and autoradiographic findings. The gross findings of the fracture revealed the followings; 1) There was more fibrous union at the fracture site in the left experimental sides than the right control sides at the 5th day except for group Ⅲ. 2) Callus appeared at the fracture site from the first week and more callus was found in the experimental sides of groups Ⅰ and Ⅱ than in the control sides. 3) There appeared less callus formation in the experimental side of group Ⅲ than in the control side at the 3rd week. 4) Bony union occured from the 4th week in all groups and no difference was noticed between the experimental and control sides from the 4th week to 7th week. The radiological findings were as follows; 1) Callus appeared from the first week and more callus was found in the experimental sides of groups Ⅰ, Ⅱ and Ⅳ than in the control sides at the 2nd week. 2) There was a clear evidence that more bony union occured in all experimental sides except group Ⅲ than in the controls at the 3rd week. 3) More bony union occured at the fracture site at the 4th week than the 3rd week in groups Ⅰ, Ⅱ and Ⅳ. 4) No difference was found between the experimental and control sides from the 5th week. The microscopic findings were as follows; 1) In all groups there appeared callus formation and more cartilage cells appeared in the experimental sides of groups Ⅰ, Ⅱ and Ⅳ than in the control sides at the 1st week. 2) There were more cartilage cells at the 2nd week than the 1st week and even calcification appeared in the experimental sides of groups Ⅰ and Ⅳ at the 2nd week. 3) From the 3rd week trabecula appeared and more trabeculae were found in the experimental sides of groups Ⅰ, Ⅱ and Ⅳ than in the control sides. 4) There was no remarkable difference between the experimental and control sides except group Ⅳ where cartilage cells were found in the control side at the 4th week. 5) No remarkable difference was found between all experimental and control sides from the 5th week. The autoradiographic findings were as follows; 1) The dark grain appeared from the 5th day and there were more grains in the experimental sides of groups Ⅱ and Ⅳ than in the control sides at the 5th day. 2) More grains appeared in all experimental sides of groups except group Ⅲ than in the control sides at the 1st week. 3) The highest incidence of grain was at the 2nd week in the groups Ⅰ and Ⅱ, and at the 3rd week in the groups Ⅲ and Ⅳ. More grains appeared in all experimetal sides of groups except group Ⅳ than in the control sides at the 2nd week. 4) There was no difference between the experimental and control sides of groups except group Ⅳ from the 3rd week. 5) There was less grain from the 4th week in all groups. These experimental results show that; 1) There were more callus formation and bony union in the experimental sides of groups Ⅰ, Ⅱ, and Ⅳ than in the control ones at the 1st, 2nd and 3rd week. 2) In group Ⅲ there were less callus formation and bony union in the experimental side than the control one at the 2nd, 3rd and 4th week. 3) There was no difference between all experimental and control sides at the 5th and 7th week. 4) The dark grain appeared from the 5th day and the frequency reached a peak at the 2nd to 3rd week from the autoradiographic findings. 5) It was found that electric current could accelerate the callus formation and bony union in the fracture.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/115176
Appears in Collections:
2. 학위논문 > 1. College of Medicine (의과대학) > 박사
Yonsei Authors
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