Cited 41 times in
Dissemination of transferable CTX-M-type extended-spectrum β-lactamase-producing Escherichia coli in Korea
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 배일권 | - |
dc.contributor.author | 정석훈 | - |
dc.date.accessioned | 2015-08-26T16:35:15Z | - |
dc.date.available | 2015-08-26T16:35:15Z | - |
dc.date.issued | 2005 | - |
dc.identifier.issn | 1364-5072 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/114747 | - |
dc.description.abstract | AIMS: Among 365 Escherichia coli isolated in 2003, 31 cefotaxime-resistant isolates were obtained from clinical specimens taken from adults hospitalized in Busan, Korea. Six extended-spectrum beta-lactamase (ESBL)-producing isolates were investigated further to determine the mechanism of resistance. METHODS AND RESULTS: These isolates were analysed by antibiotic susceptibility testing, pI determination, plasmid profiles, transconjugation test, PCR-restriction fragment length polymorphism (RFLP), enterobacterial repetitive consensus (ERIC)-PCR and DNA sequencing. All six of these isolates were found to contain the CTX-M-type ESBL genes. Five clinical isolates and their transconjugants produced CTX-M-3. One clinical isolate (K17391) and its transconjugant (trcK17391) produced CTX-M-15. Five clinical isolates also produced another TEM-1. One clinical isolate (K12776) also contained another TEM-52. CTX-M-3 ESBL gene was responsible for the resistance to piperacillin, cephalothin, cefotaxime, cefepime and aztreonam. CTX-M-15 or TEM-52 was especially responsible for the resistance to ceftazidime. CONCLUSIONS: These results appear to represent the in vivo evolution of CTX-M-type beta-lactamase genes (bla(CTX-M-3) --> bla(CTX-M-15)) under the selective pressure of antimicrobial therapy (especially ceftazidime). PCR-RFLP is a reliable method to discriminate CTX-M-15 gene from CTX-M-3 gene. ERIC-PCR analysis revealed that dissemination of CTX-M-3 was not due to a clonal outbreak of a resistant strain but to the intra-species spread of resistance to piperacillin, cephalothin, cefotaxime, cefepime and aztreonam in Korea. SIGNIFICANCE AND IMPACT OF THE STUDY: This is the first report of the occurrence of CTX-M-1 cluster ESBLs in Korea. A more comprehensive survey of these ESBL types from Korea is urgently needed because of the in vivo evolution of CTX-M-15 from CTX-M-3. The emergence of these CTX-M-type ESBLs suggests that diagnostic laboratories should screen for ESBLs with ceftazidime as well as cefotaxime; they should still perform clavulanate synergy tests on resistant isolates. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 921~927 | - |
dc.relation.isPartOf | JOURNAL OF APPLIED MICROBIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Anti-Bacterial Agents/pharmacology* | - |
dc.subject.MESH | Base Sequence | - |
dc.subject.MESH | Cefotaxime/pharmacology* | - |
dc.subject.MESH | Cephalosporin Resistance/genetics* | - |
dc.subject.MESH | Conjugation, Genetic/genetics | - |
dc.subject.MESH | DNA, Bacterial/genetics | - |
dc.subject.MESH | Escherichia coli/drug effects | - |
dc.subject.MESH | Escherichia coli/genetics* | - |
dc.subject.MESH | Escherichia coli/isolation & purification | - |
dc.subject.MESH | Genes, Bacterial/genetics | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Isoelectric Focusing/methods | - |
dc.subject.MESH | Korea | - |
dc.subject.MESH | Microbial Sensitivity Tests/methods | - |
dc.subject.MESH | Plasmids/genetics | - |
dc.subject.MESH | Polymerase Chain Reaction/methods | - |
dc.subject.MESH | Polymorphism, Restriction Fragment Length | - |
dc.subject.MESH | beta-Lactamases/genetics* | - |
dc.title | Dissemination of transferable CTX-M-type extended-spectrum β-lactamase-producing Escherichia coli in Korea | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | S.H. Jeong | - |
dc.contributor.googleauthor | I.K. Bae | - |
dc.contributor.googleauthor | S.H. Lee | - |
dc.contributor.googleauthor | S.J. Jang | - |
dc.contributor.googleauthor | K.H. Sung | - |
dc.contributor.googleauthor | H.I. Jung | - |
dc.contributor.googleauthor | J.S. Song | - |
dc.contributor.googleauthor | J.H. Lee | - |
dc.contributor.googleauthor | S.B. Kwon | - |
dc.identifier.doi | 10.1111/j.1365-2672.2004.02526.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01802 | - |
dc.contributor.localId | A03619 | - |
dc.relation.journalcode | J01238 | - |
dc.identifier.eissn | 1365-2672 | - |
dc.identifier.pmid | 15752339 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1365-2672.2004.02526.x/abstract | - |
dc.subject.keyword | b-lactamase | - |
dc.subject.keyword | CTX-M | - |
dc.subject.keyword | enterobacterial repetitive consensus-PCR | - |
dc.subject.keyword | extended-spectrum b-lactamase | - |
dc.subject.keyword | PCR-RFLP | - |
dc.contributor.alternativeName | Bae, Il Kwon | - |
dc.contributor.alternativeName | Jeong, Seok Hoon | - |
dc.contributor.affiliatedAuthor | Bae, Il Kwon | - |
dc.contributor.affiliatedAuthor | Jeong, Seok Hoon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 98 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 921 | - |
dc.citation.endPage | 927 | - |
dc.identifier.bibliographicCitation | JOURNAL OF APPLIED MICROBIOLOGY, Vol.98(4) : 921-927, 2005 | - |
dc.identifier.rimsid | 46095 | - |
dc.type.rims | ART | - |
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