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Development of a Conditional Replication Competent Adenovirus, Controlled by the Human Telomerase Promoter
DC Field | Value | Language |
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dc.contributor.author | 손주혁 | - |
dc.contributor.author | 윤채옥 | - |
dc.date.accessioned | 2015-07-15T16:55:50Z | - |
dc.date.available | 2015-07-15T16:55:50Z | - |
dc.date.issued | 2003 | - |
dc.identifier.issn | 1598-2998 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/113883 | - |
dc.description.abstract | PURPOSE: This study has been planned to generate a replication-competent adenovirus which replicates in a cancer cell-specific manner, thus minimizing the side effects and toxicity of cancer gene therapy. MATERIALS AND METHODS: we have generated an E1B 19 kD attenuated recombinant adenoviruses, Ad-TERT-delta19 and Ad-mTERT-delta19, which encode E1A gene driven by the wild type hTERT and modified m-hTERT promoter containing additional c-myc and Sp1 binding sites in the backbone of Ad-deltaE1B19. The in vitro efficacy and specificity of the hTERT and m-hTERT promoter have been evaluated by the comparison of viral replication and cytopathic effect in cancer cells and normal cell lines. To assess anti-tumor effect and safety of hTERT or m-hTERT promoter driven replication competent adenoviruses, tumor regression after subcutaneous injection in subcutaneous C33A xenografts and lacZ expression after systemic injection in organs were examined. RESULTS: The activation of hTERT or m-hTERT promoter was significantly up-regulated only in hTERT-positive cells, but not in hTERT-negative cells. Moreover, the activity of m-hTERT promoter was substantially increased in hTERT-positive cancer cells, but not in hTERT-negative cells. While Ad-TERT-delta19 replicated in and induced cytopathic effect in cancer and in some normal cell lines, Ad-mTERT-delta19 enhanced viral replication and cytopathic effect in cancer cells only. Furthermore, the growth of established human cervical carcinoma in nude mice was significantly suppressed by intratumoral injection of Ad-mTERT-delta19. CONCLUSIONS: The use of m-hTERT promoter is not only useful in the regulation of therapeutic gene expression but also that replication-competent oncolytic adenovirus under the control of m-hTERT promoter may be a new promising tool for the treatment of human malignancies. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 191~206 | - |
dc.relation.isPartOf | CANCER RESEARCH AND TREATMENT | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cancer gene therapy | - |
dc.subject.MESH | Replication competent adenovirus | - |
dc.subject.MESH | hTERT | - |
dc.title | Development of a Conditional Replication Competent Adenovirus, Controlled by the Human Telomerase Promoter | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Institute for Cancer Research (암연구소) | - |
dc.contributor.googleauthor | Eunhee Kim | - |
dc.contributor.googleauthor | Joo Hang Kim | - |
dc.contributor.googleauthor | Chae Ok Yun | - |
dc.contributor.googleauthor | Jungho Kim | - |
dc.contributor.googleauthor | Jai Myung Yang | - |
dc.contributor.googleauthor | Joo Hyuk Sohn | - |
dc.contributor.googleauthor | Han Saem Lee | - |
dc.contributor.googleauthor | Ha Youn Shin | - |
dc.identifier.doi | 10.4143/crt.2003.35.3.191 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01995 | - |
dc.contributor.localId | A02614 | - |
dc.relation.journalcode | J00453 | - |
dc.identifier.eissn | 2005-9256 | - |
dc.identifier.pmid | 26680936 | - |
dc.subject.keyword | Cancer gene therapy | - |
dc.subject.keyword | Replication competent adenovirus | - |
dc.subject.keyword | hTERT | - |
dc.contributor.alternativeName | Sohn, Joo Hyuk | - |
dc.contributor.alternativeName | Yun, Chae Ok | - |
dc.contributor.affiliatedAuthor | Sohn, Joo Hyuk | - |
dc.contributor.affiliatedAuthor | Yun, Chae Ok | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 35 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 191 | - |
dc.citation.endPage | 206 | - |
dc.identifier.bibliographicCitation | CANCER RESEARCH AND TREATMENT, Vol.35(3) : 191-206, 2003 | - |
dc.identifier.rimsid | 41459 | - |
dc.type.rims | ART | - |
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