Facilitated forward chemical genetics using a tagged triazine library and zebrafish embryo screening
Authors
Sonya M. Khersonsky ; Da-Woon Jung ; Young-Tae Chang ; Thomas A. Neubert ; Vivekananda Shetty ; Eric M. Jacobson ; Hakryul Jo ; Ho-Sang Moon ; Daniel P. Walsh ; Tae-Wook Kang
Citation
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol.125(39) : 11804-11805, 2003
An improved forward chemical genetics approach was successfully demonstrated using a tagged library concept. A small-molecule triazine library with linkers was used to screen for brain/eye developmental phenotypes in a zebrafish embryo system. This approach enabled the rapid isolation of the target proteins by facile affinity matrix preparation and elucidated the first small-molecule inhibitors for several ribosomal accessory proteins or their complex as the target.