Background and Objectives : The purpose of this study is to analyze the difference of Fundamental Frequency(Hz), Closed Quotient(Qx ; %), Intensity(dB), Vocal tract length and width(cm), formant frequency(Hz), level of formant frequency(dB) depending on the larynx position. Materials and Methods : One professional male singer(career : 28 years) produced sustained vowel /a/,/e/,/i/,/o/,/u/ in two larynx position (higher, lower) with Dr. Speech and video fluoroscopy was used to quantify the vocal tract morphology. Results : In lower larynx position, CQ is increased 9.8% and Intensity is increased about 10% and level of Formant Frequency is increased. And also Vocal tract length is longer 2.4cm, Vocal tract width(Anterior width : 0.4cm, lateral width : 0.2cm) is wider than in higher larynx position. Conclusions : Singer's formant has a prominent spectrum envelope peak near 2400-2600Hz by clustering of F3, F4 and F5 near 3400Hz in lower larynx position.