Cited 21 times in
Cellular stress-related protein expression in Helicobacter pylori-infected gastric epithelial AGS cells
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.contributor.author | 임주원 | - |
dc.date.accessioned | 2015-07-14T16:50:58Z | - |
dc.date.available | 2015-07-14T16:50:58Z | - |
dc.date.issued | 2004 | - |
dc.identifier.issn | 1357-2725 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/111712 | - |
dc.description.abstract | Helicobacter pylori infection leads to gastroduodenal inflammation, peptic ulceration, and gastric carcinoma. Moreover, H. pylori may induce disease-specific protein expression in gastric epithelial cells. The present study was aimed at determining differentially expressed proteins in H. pylori-infected gastric epithelial AGS cells. AGS cells were treated with H. pylori at a bacterium/cell ratio of 300:1 for 12 h. Altered protein patterns as separated by two-dimensional electrophoresis using pH gradients of 4-7 were conclusively identified by matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) analysis of the peptide digests. Four differentially expressed proteins, whose expression levels were increased by more than two-fold in H. pylori-infected cells, were analyzed. These proteins (14-3-3 protein alpha/beta, cullin homolog 3, alpha-enolase, ezrin) are known to be related to cell proliferation, cell adhesion, and carcinogenesis, and may be mediated by cellular stress, such as reactive oxygen species. In conclusion, the identification of these differentially expressed proteins provide valuable information for the understanding of the pathophysiologic mechanisms of H. pylori-induced gastric diseases, and may be useful as prognostic indices of H. pylori-related gastric disorders. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1624~1634 | - |
dc.relation.isPartOf | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Electrophoresis, Gel, Two-Dimensional | - |
dc.subject.MESH | Epithelial Cells/metabolism | - |
dc.subject.MESH | Epithelial Cells/microbiology* | - |
dc.subject.MESH | Gastric Mucosa/metabolism | - |
dc.subject.MESH | Gastric Mucosa/microbiology* | - |
dc.subject.MESH | Helicobacter Infections/pathology* | - |
dc.subject.MESH | Helicobacter pylori* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Oxidative Stress* | - |
dc.subject.MESH | Proteins/analysis | - |
dc.subject.MESH | Proteins/genetics | - |
dc.subject.MESH | Proteomics/methods | - |
dc.subject.MESH | Reactive Oxygen Species | - |
dc.subject.MESH | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | - |
dc.subject.MESH | Up-Regulation/physiology* | - |
dc.title | Cellular stress-related protein expression in Helicobacter pylori-infected gastric epithelial AGS cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Joo Weon Lim | - |
dc.contributor.googleauthor | Hyeyoung Kim | - |
dc.contributor.googleauthor | Kyung Hwan Kim | - |
dc.contributor.googleauthor | Hyun Chae Jung | - |
dc.contributor.googleauthor | Joo Sung Kim | - |
dc.contributor.googleauthor | Jung Mogg Kim | - |
dc.identifier.doi | 10.1016/j.biocel.2004.01.018 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.relation.journalcode | J01089 | - |
dc.identifier.eissn | 1878-5875 | - |
dc.identifier.pmid | 15147740 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1357272504000329 | - |
dc.subject.keyword | Helicobacter pylori | - |
dc.subject.keyword | Gastric epithelial cells | - |
dc.subject.keyword | Proteome | - |
dc.subject.keyword | Cellular stress | - |
dc.subject.keyword | Reactive oxygen species | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.alternativeName | Lim, Joo Weon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 36 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1624 | - |
dc.citation.endPage | 1634 | - |
dc.identifier.bibliographicCitation | INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Vol.36(8) : 1624-1634, 2004 | - |
dc.identifier.rimsid | 37428 | - |
dc.type.rims | ART | - |
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