Cited 4 times in
Generation of a rabbit VH domain antibody polyspecific to c-Met and adenoviral knob protein
DC Field | Value | Language |
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dc.contributor.author | 김주항 | - |
dc.contributor.author | 윤채옥 | - |
dc.date.accessioned | 2015-06-10T12:49:40Z | - |
dc.date.available | 2015-06-10T12:49:40Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0006-291X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/110517 | - |
dc.description.abstract | Several types of bispecific antibodies with affinity to both adenoviral coat proteins and a targeted antigen have been developed with the aim of providing the specific delivery of adenoviral gene therapy vehicle. From a phage display library of combinatorial dAb2s (each with an anti-adenoviral knob protein VH fragment linked with an anti-c-Met VH), we serendipitously enriched and isolated a clone, JS5, that has polyspecificity such that it binds both the adenoviral knob protein and c-Met, despite having only one VH domain. Our indirect observations suggest that the polyspecificity of JS5 is developed through accumulation of antibody specificity. The method of sequential immunization of a rabbit, first with the adenoviral knob protein and then with target antigens, may provide a method by which monoclonal antibodies with stand-alone polyspecificity may be developed. Such targeted polyspecific antibodies could readily be used for re-directing adenoviral vectors to target cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 305~312 | - |
dc.relation.isPartOf | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenoviridae/immunology* | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antibodies, Bispecific* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoglobulin Heavy Chains/immunology* | - |
dc.subject.MESH | Immunoglobulin Variable Region/immunology* | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Peptide Library | - |
dc.subject.MESH | Proto-Oncogene Proteins c-met/immunology* | - |
dc.subject.MESH | Rabbits | - |
dc.subject.MESH | Viral Proteins/immunology* | - |
dc.title | Generation of a rabbit VH domain antibody polyspecific to c-Met and adenoviral knob protein | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Institute for Cancer Research (암연구소) | - |
dc.contributor.googleauthor | Shin-Young Im | - |
dc.contributor.googleauthor | Ki Su Kim | - |
dc.contributor.googleauthor | Chae-Ok Yun | - |
dc.contributor.googleauthor | Joo-Hang Kim | - |
dc.contributor.googleauthor | Kye-Sook Yi | - |
dc.contributor.googleauthor | Junho Chung | - |
dc.identifier.doi | 10.1016/j.bbrc.2005.10.208 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00945 | - |
dc.contributor.localId | A02614 | - |
dc.relation.journalcode | J00281 | - |
dc.identifier.eissn | 1090-2104 | - |
dc.identifier.pmid | 16298341 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006291X05025192 | - |
dc.subject.keyword | Bispecific antibody | - |
dc.subject.keyword | Domain antibody | - |
dc.subject.keyword | c-Met | - |
dc.subject.keyword | Adenoviral knob protein | - |
dc.contributor.alternativeName | Kim, Joo Hang | - |
dc.contributor.alternativeName | Yun, Chae Ok | - |
dc.contributor.affiliatedAuthor | Kim, Joo Hang | - |
dc.contributor.affiliatedAuthor | Yun, Chae Ok | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 339 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 305 | - |
dc.citation.endPage | 312 | - |
dc.identifier.bibliographicCitation | BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, Vol.339(1) : 305-312, 2006 | - |
dc.identifier.rimsid | 39225 | - |
dc.type.rims | ART | - |
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