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Antigen discovery: A postgenomic approach to leprosy diagnosis

Authors
 Romulo Aráoz  ;  Nadine Honoré  ;  Sungae Cho  ;  Jong-Pill Kim  ;  Sang-Nae Cho  ;  Marc Monot  ;  Caroline Demangel  ;  Patrick J. Brennan  ;  Stewart T. Cole 
Citation
 INFECTION AND IMMUNITY, Vol.74(1) : 175-182, 2006 
Journal Title
INFECTION AND IMMUNITY
ISSN
 0019-9567 
Issue Date
2006
MeSH
Amino Acid Sequence ; Antibodies, Bacterial/biosynthesis ; Antibodies, Bacterial/blood ; Antigens, Bacterial/biosynthesis ; Antigens, Bacterial/genetics ; Antigens, Bacterial/immunology* ; Computational Biology ; Genes, Bacterial ; Genome, Bacterial* ; Humans ; Interferon-gamma/metabolism ; Leprosy/blood ; Leprosy/diagnosis* ; Leprosy/immunology ; Leprosy/metabolism ; Molecular Sequence Data ; Mycobacterium leprae/immunology*
Abstract
Leprosy is an infectious, neurodegenerative disease of humans caused by Mycobacterium leprae. Despite effective control programs, the incidence of leprosy remains stubbornly high, suggesting that transmission may be more common than expected. The rationale of this work was to use bioinformatics and comparative genomics to identify potentially antigenic proteins for diagnostic purposes. This approach defined three classes of proteins: those restricted to M. leprae (class I), those present in M. leprae with orthologues in other organisms besides mycobacteria (class II), and exported or surface-exposed proteins (class III). Twelve genes (two class I, four class II, and six class III proteins) were cloned in Escherichia coli, and their protein products were purified. Six of these proteins were detected in cell extracts of M. leprae by immunoblotting. The immunogenicity of each recombinant protein was then investigated in leprosy patients by measuring the reactivity of circulating antibody and gamma interferon (IFN-γ) responses in T-cell restimulation assays. Several class II and class III proteins were recognized by circulating antibodies. Importantly, most class II proteins elicited IFN-γ responses that were significantly stronger than those produced by previously identified antigens. Among them, two class II proteins, ML0308 and ML2498, showed marked humoral and cellular immunogenicity, therefore providing promising candidates for the diagnosis of both tuberculoid and lepromatous forms of leprosy.
Files in This Item:
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DOI
10.1128/IAI.74.1.175-182.2006
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers
Yonsei Authors
Cho, Sang Nae(조상래)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/109363
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