Cited 4 times in
Efficient cellular uptake of recombinant murine Hoxc8 homeoprotein in COS-7 cells
DC Field | Value | Language |
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dc.contributor.author | 김명희 | - |
dc.date.accessioned | 2015-06-10T12:04:42Z | - |
dc.date.available | 2015-06-10T12:04:42Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0024-3205 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/109150 | - |
dc.description.abstract | In order to analyze the self-delivery activity of Hoxc8, recombinant Hoxc8 protein (rHoxc8) was designed to be expressed and purified in E. coli as a glutathione S-transferase and green fluorescent protein-fused form (GST-GFP-Hoxc8). After purification using glutathione sepharose beads, the 82 kDa fusion protein was separated on the SDS-PAGE gel and confirmed by detecting the fluorescence through luminescent image analyzer. When rHoxc8 was added to culture media for 30 h, most of the COS-7 cells contained the fusion proteins, showing green fluorescence under the fluorescent microscope. When the efficiency of cellular uptake was examined after Hoechst staining, almost 100% of the cells exhibited the GFP signal, revealing that rHoxc8 can traverse the cellular membrane of COS-7 cells efficiently, suggesting that the rHoxc8 could be applied in the development of efficient and useful delivery vectors for therapeutic molecules. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 2345~2348 | - |
dc.relation.isPartOf | LIFE SCIENCES | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | COS Cells/metabolism | - |
dc.subject.MESH | Cercopithecus aethiops | - |
dc.subject.MESH | Electrophoresis, Polyacrylamide Gel | - |
dc.subject.MESH | Escherichia coli/genetics | - |
dc.subject.MESH | Escherichia coli/metabolism | - |
dc.subject.MESH | Fluorescence | - |
dc.subject.MESH | Glutathione Transferase/genetics | - |
dc.subject.MESH | Glutathione Transferase/metabolism | - |
dc.subject.MESH | Green Fluorescent Proteins/genetics | - |
dc.subject.MESH | Green Fluorescent Proteins/metabolism | - |
dc.subject.MESH | Homeodomain Proteins/genetics | - |
dc.subject.MESH | Homeodomain Proteins/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Recombinant Fusion Proteins/genetics | - |
dc.subject.MESH | Recombinant Fusion Proteins/metabolism* | - |
dc.subject.MESH | Transduction, Genetic | - |
dc.title | Efficient cellular uptake of recombinant murine Hoxc8 homeoprotein in COS-7 cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anatomy (해부학) | - |
dc.contributor.googleauthor | Mi Hee Lee | - |
dc.contributor.googleauthor | Hyoung Woo Park | - |
dc.contributor.googleauthor | Myoung Hee Kim | - |
dc.identifier.doi | 10.1016/j.lfs.2006.07.039 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00432 | - |
dc.relation.journalcode | J02167 | - |
dc.identifier.eissn | 1879-0631 | - |
dc.identifier.pmid | 16949104 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0024320506006072 | - |
dc.subject.keyword | Hoxc8 | - |
dc.subject.keyword | Homeoprotein | - |
dc.subject.keyword | Protein transduction | - |
dc.subject.keyword | Delivery vector | - |
dc.subject.keyword | COS-7 cells | - |
dc.contributor.alternativeName | Kim, Myoung Hee | - |
dc.contributor.affiliatedAuthor | Kim, Myoung Hee | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 79 | - |
dc.citation.number | 25 | - |
dc.citation.startPage | 2345 | - |
dc.citation.endPage | 2348 | - |
dc.identifier.bibliographicCitation | LIFE SCIENCES, Vol.79(25) : 2345-2348, 2006 | - |
dc.identifier.rimsid | 50624 | - |
dc.type.rims | ART | - |
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