Cited 12 times in
Identification of HLA-A33-restricted CMV pp65 epitopes as common targets for CD8+ CMV-specific cytotoxic T lymphocytes
DC Field | Value | Language |
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dc.contributor.author | 권오헌 | - |
dc.contributor.author | 임종백 | - |
dc.date.accessioned | 2015-06-10T11:52:11Z | - |
dc.date.available | 2015-06-10T11:52:11Z | - |
dc.date.issued | 2006 | - |
dc.identifier.issn | 0301-472X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/108768 | - |
dc.description.abstract | OBJECTIVE: To identify an immunodominant HLA-A33-restricted epitope within the CMV matrix phosphoprotein 65 (pp65) that could be used to elicit CMV-specific CTLs. METHODS: A computer algorithm was used to identify pp65 peptides that were expected to bind to HLA-A33. The peptides were screened for their ability to reactivate memory T lymphocytes from CMV-seropositive HLA-A33 donors by using quantitative real-time RT-PCR. The most promising peptides were then tested for their ability to expand a CD8(+) population of HLA-A33 CTLs that produced interferon-gamma (IFN-gamma) and were cytotoxic to either peptide-loaded or CMV-infected target cells. RESULTS: Sixteen out of 250 peptides were selected using a computer algorithm and peptide stimulation by 8 out of the 16 induced a significant quantity of IFN-gamma mRNA transcript from CMV-seropositive HLA-A33 peripheral blood mononuclear cells. All the eight peptides induced consistent T-cell reactivation. One specifically, the peptide pp65(91-100) (SVNVHNPTGR), proved to be more active. T cells in vitro sensitized for 2 or 3 weeks with pp65(91-100) were cytotoxic to both HLA-A33 peptide-loaded and CMV-infected target cells. CONCLUSIONS: CMV pp65(91-100) is a new HLA-A33-restricted peptide that broadens the list of antigenic determinants that can be used for CMV adoptive immunotherapy. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 296~307 | - |
dc.relation.isPartOf | EXPERIMENTAL HEMATOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Amino Acid Sequence | - |
dc.subject.MESH | CD8-Positive T-Lymphocytes/immunology* | - |
dc.subject.MESH | Epitopes/chemistry | - |
dc.subject.MESH | Epitopes/immunology* | - |
dc.subject.MESH | Flow Cytometry | - |
dc.subject.MESH | HLA-A Antigens/immunology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunologic Memory | - |
dc.subject.MESH | Interferon-gamma/biosynthesis | - |
dc.subject.MESH | Interferon-gamma/genetics | - |
dc.subject.MESH | Molecular Sequence Data | - |
dc.subject.MESH | Phosphoproteins/immunology* | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.subject.MESH | Reverse Transcriptase Polymerase Chain Reaction | - |
dc.subject.MESH | Viral Matrix Proteins/immunology* | - |
dc.title | Identification of HLA-A33-restricted CMV pp65 epitopes as common targets for CD8+ CMV-specific cytotoxic T lymphocytes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Laboratory Medicine (진단검사의학) | - |
dc.contributor.googleauthor | Jong-Baeck Lim | - |
dc.contributor.googleauthor | Maurizio Provenzano | - |
dc.contributor.googleauthor | Oh Hun Kwon | - |
dc.contributor.googleauthor | Maria Bettinotti | - |
dc.contributor.googleauthor | Lorraine Caruccio | - |
dc.contributor.googleauthor | Dirk Nagorsen | - |
dc.contributor.googleauthor | David Stroncek | - |
dc.identifier.doi | 10.1016/j.exphem.2005.12.006 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00237 | - |
dc.contributor.localId | A03403 | - |
dc.relation.journalcode | J00870 | - |
dc.identifier.eissn | 1873-2399 | - |
dc.identifier.pmid | 16543064 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0301472X05005679 | - |
dc.contributor.alternativeName | Kwon, Oh Hun | - |
dc.contributor.alternativeName | Lim, Jong Baeck | - |
dc.contributor.affiliatedAuthor | Kwon, Oh Hun | - |
dc.contributor.affiliatedAuthor | Lim, Jong Baeck | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 34 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 296 | - |
dc.citation.endPage | 307 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL HEMATOLOGY, Vol.34(3) : 296-307, 2006 | - |
dc.identifier.rimsid | 57688 | - |
dc.type.rims | ART | - |
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