Cited 20 times in
Epigallocatechin-3-gallate inhibits interleukin-1beta-induced MUC5AC gene expression and MUC5AC secretion in normal human nasal epithelial cells.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 윤주헌 | - |
dc.contributor.author | 이정권 | - |
dc.date.accessioned | 2015-05-19T17:10:07Z | - |
dc.date.available | 2015-05-19T17:10:07Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0955-2863 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/107631 | - |
dc.description.abstract | It has been reported that the proinflammatory cytokine interleukin-1beta (IL-1beta) induces mucus hypersecretion in normal human nasal epithelial (NHNE) cells and that the MAP kinase pathway may be an important signal pathway in IL-1beta-induced MUC5AC gene expression. Green tea (Camellia sinensis) polyphenols are potent anti-inflammatory agents and have been shown to inhibit inflammation in tumor cell lines and cultured respiratory epithelial cells. In this study, we examined the effect of (-)-epigallocatechin-3-gallate (EGCG), a green tea polyphenol, on IL-1beta-induced MUC5AC gene expression and secretion in NHNE cells. After cells had been treated with IL-1beta (10 ng/ml) and pretreated with EGCG (10, 50 and 100 microM), mRNA expression of MUC5AC was determined by real-time polymerase chain reaction. The suppression of each signal pathway protein was determined by Western blot analysis after treatment with IL-1beta and EGCG, respectively. IL-1beta increased MUC5AC gene expression and MUC5AC secretion. EGCG markedly suppressed IL-1beta-induced MUC5AC gene expression and MUC5AC secretion via suppression of the phosphorylation of ERK MAP kinase, MSK1, and transcription factor, cAMP response element-binding protein. IL-1beta increased the number of cells staining positive with MUC5AC antibodies, and EGCG treatment decreased this number. Our data suggest that EGCG may be an effective inhibitor of IL-1beta-induced mucus hypersecretion. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 536~544 | - |
dc.relation.isPartOf | JOURNAL OF NUTRITIONAL BIOCHEMISTRY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Catechin/analogs & derivatives* | - |
dc.subject.MESH | Catechin/pharmacology | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Enzyme Activation/drug effects | - |
dc.subject.MESH | Gene Expression/drug effects* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-1beta/antagonists & inhibitors* | - |
dc.subject.MESH | Interleukin-1beta/pharmacology | - |
dc.subject.MESH | Lung Neoplasms | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 1/metabolism | - |
dc.subject.MESH | Mitogen-Activated Protein Kinase 3/metabolism | - |
dc.subject.MESH | Mucin 5AC | - |
dc.subject.MESH | Mucins/genetics* | - |
dc.subject.MESH | Mucins/metabolism* | - |
dc.subject.MESH | Nasal Mucosa/drug effects* | - |
dc.subject.MESH | Nasal Mucosa/metabolism | - |
dc.subject.MESH | Promoter Regions, Genetic/drug effects | - |
dc.subject.MESH | Signal Transduction/drug effects | - |
dc.subject.MESH | Tumor Cells, Cultured | - |
dc.title | Epigallocatechin-3-gallate inhibits interleukin-1beta-induced MUC5AC gene expression and MUC5AC secretion in normal human nasal epithelial cells. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Otorhinolaryngology (이비인후과학) | - |
dc.contributor.googleauthor | Hyun Jik Kim | - |
dc.contributor.googleauthor | Sang Ho Park | - |
dc.contributor.googleauthor | Sung-Yoon Park | - |
dc.contributor.googleauthor | Uk Yeol Moon | - |
dc.contributor.googleauthor | Byung Don Lee | - |
dc.contributor.googleauthor | Sung Hyun Yoon | - |
dc.contributor.googleauthor | Jeung-Gweon Lee | - |
dc.contributor.googleauthor | Seung Joon Baek | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.identifier.doi | 10.1016/j.jnutbio.2007.06.010 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02604 | - |
dc.contributor.localId | A03095 | - |
dc.relation.journalcode | J01650 | - |
dc.identifier.eissn | 1873-4847 | - |
dc.identifier.pmid | 18155512 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0955286307001805 | - |
dc.subject.keyword | EGCG | - |
dc.subject.keyword | IL-1β | - |
dc.subject.keyword | MUC5AC | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.alternativeName | Lee, Jeung Gweon | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Lee, Jeung Gweon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 19 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 536 | - |
dc.citation.endPage | 544 | - |
dc.identifier.bibliographicCitation | JOURNAL OF NUTRITIONAL BIOCHEMISTRY, Vol.19(8) : 536-544, 2008 | - |
dc.identifier.rimsid | 54800 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.