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Development of PCR-microplate Hybridization Assay for Detection of Mycobacterium tuberculosis
DC Field | Value | Language |
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dc.contributor.author | 조상래 | - |
dc.date.accessioned | 2015-04-24T17:40:08Z | - |
dc.date.available | 2015-04-24T17:40:08Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1738-3226 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/105839 | - |
dc.description.abstract | Tuberculosis caused by Mycobacterium tuberculosis (MTB) still remains to be the most dreadful infectious disease affecting almost every country. In the present study, we developed a simple and rapid but accurate and sensitive assay method for detecting MTB using microplate hybridization assay. For this, a selective region of the rpoB gene was used to design PCR primers, and MTB and Mycobacterium genus-specific probe molecules. The specificity of the assay was confirmed using fifteen different mycobacterial reference strains and twelve different non-mycobacterial reference strains, and the sensitivity was determined to be 100 fg using genomic DNA of MTB reference strain, H37Rv. Subsequently, a total of 62 sputum samples with diverse smear scores and culture positive results were used to evaluate the kit performance. In brief, the specificity and the sensitivity of the assay were 100% and 98.4%, respectively | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 295~300 | - |
dc.relation.isPartOf | Journal of Experimental & Biomedcal Science (대한의생명과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Development of PCR-microplate Hybridization Assay for Detection of Mycobacterium tuberculosis | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | In Soo Lee | - |
dc.contributor.googleauthor | Een Jin Cho | - |
dc.contributor.googleauthor | Sang-Nae Cho | - |
dc.contributor.googleauthor | Tae Ue Kim | - |
dc.contributor.googleauthor | Hye Young Lee | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03824 | - |
dc.relation.journalcode | J01408 | - |
dc.subject.keyword | Mycobacterium tuberculosis | - |
dc.subject.keyword | Microplate hybridization assay | - |
dc.subject.keyword | Nontuberculosis mycobacteria | - |
dc.subject.keyword | PCR | - |
dc.contributor.alternativeName | Cho, Sang Nae | - |
dc.contributor.affiliatedAuthor | Cho, Sang Nae | - |
dc.citation.volume | 15 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 295 | - |
dc.citation.endPage | 300 | - |
dc.identifier.bibliographicCitation | Journal of Experimental & Biomedcal Science (대한의생명과학회지), Vol.15(4) : 295-300, 2009 | - |
dc.identifier.rimsid | 49880 | - |
dc.type.rims | ART | - |
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