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고위험병원체 결핵균의 신속진단을 위한 액체배양시스템 평가

Other Titles
 Evaluation of Liquid Culture System in Sputum Culture and Drug Susceptibility of Mycobacterium tuberculosis 
Authors
 김진숙  ;  김승철  ;  전보영  ;  박승규 
Citation
 Korean Journal of Microbiology (미생물학회지), Vol.45(3) : 281-285, 2009 
Journal Title
Korean Journal of Microbiology(미생물학회지)
ISSN
 0440-2413 
Issue Date
2009
Keywords
acid fast bacilli (AFB) ; BacT/alert process system ; isoniazid (INH) ; M. tubercilosis ; rifampicin (RIF) ; sputum culture ; drug susceptibility test (DST)
Abstract
In this study, we compared the BacT/Alert liquid culture system with Ogawa and $L\ddot{o}wenstein$-Jensen (L-J) media for sputum culture and drug susceptibility test (DST) of Mycobacterium tuberculosis. Rapid liquid culture systems have been widely employed both for primary cultures of M. tuberculosis from clinical specimens and for drug susceptibility test because of its greater sensitivity and faster turn-around time than the conventional egg-based culture methods (Ogawa, $L\ddot{o}wenstein$-Jensen media). Sputum specimens were decontaminated with N-acetyl-L-cysteine (NALC)-4% NaOH and inoculated into the BacT/Alert culture bottles and Ogawa media. 95 from among 135 sputa were smear-positive, 97 (71.9%) were culture-positive by the BacT/Alert culture system, while 89 (65.9%) were positive by Ogawa media. The mean time to culture-positive by the BacT/Alert process system was about 11.3 days, which was significantly shorter than that by Ogawa media (22.4 days). Of 32 M. tuberculosis cultures examined for drug sensitivity, the concordant rate between the two methods (BacT/ Alert liquid culture system, $L\ddot{o}wenstein$-Jensen media) ranged from 87.5% for isoniazid and 90.6% for rifampicin.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > Research Institute (부설연구소) > 1. Journal Papers
Yonsei Authors
Jeon, Bo Young(전보영)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/105822
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