Cited 54 times in
Xenon preconditioning: the role of prosurvival signaling, mitochondrial permeability transition and bioenergetics in rats.
DC Field | Value | Language |
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dc.contributor.author | 심연희 | - |
dc.date.accessioned | 2015-04-24T16:57:54Z | - |
dc.date.available | 2015-04-24T16:57:54Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0003-2999 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/104503 | - |
dc.description.abstract | BACKGROUND: Similar to volatile anesthetics, the anesthetic noble gas xenon protects the heart from ischemia/reperfusion injury, but the mechanisms responsible for this phenomenon are not fully understood. We tested the hypothesis that xenon-induced cardioprotection is mediated by prosurvival signaling kinases that target mitochondria. METHODS: Male Wistar rats instrumented for hemodynamic measurements were subjected to a 30 min left anterior descending coronary artery occlusion and 2 h reperfusion. Rats were randomly assigned to receive 70% nitrogen/30% oxygen (control) or three 5-min cycles of 70% xenon/30% oxygen interspersed with the oxygen/nitrogen mixture administered for 5 min followed by a 15 min memory period. Myocardial infarct size was measured using triphenyltetrazolium staining. Additional hearts from control and xenon-pretreated rats were excised for Western blotting of Akt and glycogen synthase kinase 3 beta (GSK-3beta) phosphorylation and isolation of mitochondria. Mitochondrial oxygen consumption before and after hypoxia/reoxygenation and mitochondrial permeability transition pore opening were determined. RESULTS: Xenon significantly (P < 0.05) reduced myocardial infarct size compared with control (32 +/- 4 and 59% +/- 4% of the left ventricular area at risk; mean +/- sd) and enhanced phosphorylation of Akt and GSK-3beta. Xenon pretreatment preserved state 3 respiration of isolated mitochondria compared with the results obtained in the absence of the gas. The Ca(2+) concentration required to induce mitochondrial membrane depolarization was larger in the presence compared with the absence of xenon pretreatment (78 +/- 17 and 56 +/- 17 microM, respectively). The phosphoinositol-3-kinase-kinase inhibitor wortmannin blocked the effect of xenon on infarct size and respiration. CONCLUSIONS: These results indicate that xenon preconditioning reduces myocardial infarct size, phosphorylates Akt, and GSK-3beta, preserves mitochondrial function, and inhibits Ca(2+)-induced mitochondrial permeability transition pore opening. These data suggest that xenon-induced cardioprotection occurs because of activation of prosurvival signaling that targets mitochondria and renders them less vulnerable to ischemia-reperfusion injury | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 858~866 | - |
dc.relation.isPartOf | ANESTHESIA AND ANALGESIA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Anesthetics, Inhalation/pharmacology* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Blood Pressure/drug effects | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Calcium/pharmacology | - |
dc.subject.MESH | Cardiotonic Agents* | - |
dc.subject.MESH | Energy Metabolism/drug effects* | - |
dc.subject.MESH | Glycogen Synthase Kinase 3/metabolism | - |
dc.subject.MESH | Glycogen Synthase Kinase 3 beta | - |
dc.subject.MESH | Heart Rate/drug effects | - |
dc.subject.MESH | Ischemic Preconditioning, Myocardial* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mitochondria, Heart/drug effects* | - |
dc.subject.MESH | Mitochondria, Heart/pathology | - |
dc.subject.MESH | Myocardial Infarction/pathology | - |
dc.subject.MESH | Myocardial Infarction/prevention & control | - |
dc.subject.MESH | Myocardial Reperfusion Injury/pathology | - |
dc.subject.MESH | Myocardial Reperfusion Injury/prevention & control* | - |
dc.subject.MESH | Oxygen Consumption/drug effects | - |
dc.subject.MESH | Permeability/drug effects | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Proto-Oncogene Proteins c-akt/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Wistar | - |
dc.subject.MESH | Xenon/pharmacology* | - |
dc.title | Xenon preconditioning: the role of prosurvival signaling, mitochondrial permeability transition and bioenergetics in rats. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Anesthesiology (마취통증의학) | - |
dc.contributor.googleauthor | Yasushi Mio | - |
dc.contributor.googleauthor | Yon Hee shim | - |
dc.contributor.googleauthor | Ebony Richards | - |
dc.contributor.googleauthor | Zeljko J. Bosnjak | - |
dc.contributor.googleauthor | Paul S. Pagel | - |
dc.contributor.googleauthor | Martin Bienengraeber | - |
dc.identifier.doi | 10.1213/ane.0b013e318192a520 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02196 | - |
dc.relation.journalcode | J00144 | - |
dc.identifier.eissn | 1526-7598 | - |
dc.identifier.pmid | 19224794 | - |
dc.contributor.alternativeName | Shim, Yon Hee | - |
dc.contributor.affiliatedAuthor | Shim, Yon Hee | - |
dc.citation.volume | 108 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 858 | - |
dc.citation.endPage | 866 | - |
dc.identifier.bibliographicCitation | ANESTHESIA AND ANALGESIA, Vol.108(3) : 858-866, 2009 | - |
dc.identifier.rimsid | 54169 | - |
dc.type.rims | ART | - |
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