백서 두개골 및 하악골 결손에서 bovine collagen matrix의 골형성 효과

Abstract

Bovine collagen matrix used in this study consists of a porous collagen matrix of bovine origin(spongiosa) and has been evaluated as potential candidates for bone regenerative therapy. It has been used in the oral cavity under a variety of indications. This study was performed to evaluate the bone formation effect of the bovine collagen matrix in rat calvarial and mandibular defects. Critical-sized eight-mm calvarial defects and five-mm mandibular defects were created in 60 male Sprague-Dawley rats using trephine bur. The animals were divided into 4 groups. Each group received one of the following: Control(Sham-surgery), and experimental(bovine collagen matrix) for calvarial defect and mandibular defects. In the mandibular defect model, both sides of the mandible were used for the experiment. Defects were evaluated by histologic and histometric parameters following 2- and 8-week healing intervals (10 animal/group/healing interval). Overall results were uneventful without any defect exposure or inflammation. The amount of new bone formation and bone maturity increased with the increase in healing period at each group. On histologic observation, a large amount of newly formed bone was observed in the experimental group. Moreover, bovine collagen matrix was observed at 2 weeks, but by 8 weeks, the bovine collagen matrix appeared to be completely absorbed. Histometric analysis revealed that the amount of new bone was significantly greater in the collagen matrix treated sites than in the control at 8 weeks in calvarial defects (P<0.05). Within the limits of the study, these results suggest that the use of the bovine collagen matrix on the calvarial and mandibular defects in rats has a beneficial effect on the regeneration of bone tissue.