Cited 72 times in
Simple and highly effective DNA extraction methods from old skeletal remains using silica columns
DC Field | Value | Language |
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dc.contributor.author | 김나영 | - |
dc.contributor.author | 박명진 | - |
dc.contributor.author | 신경진 | - |
dc.contributor.author | 심정은 | - |
dc.contributor.author | 이환영 | - |
dc.date.accessioned | 2015-04-23T17:02:09Z | - |
dc.date.available | 2015-04-23T17:02:09Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 1872-4973 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/101701 | - |
dc.description.abstract | The recovery of DNA data from old skeletal remains is often difficult due to degraded and very low yield of extracted DNA and the presence of PCR inhibitors. Herein, we compared several silica-based DNA extraction methods from artificially degraded DNA, DNA with PCR inhibitors and DNA from old skeletal remains using quantitative real-time PCR. We present a modified large-scale silica-based extraction combined with complete demineralization, that enables maximum DNA recovery and efficient elimination of PCR inhibitors. This is performed with high concentration of EDTA solution for demineralization of bone powder followed by QIAamp spin columns and buffers from the QIAquick PCR purification kit. We have successfully used this modified technique to perform STR analysis for 55-year-old skeletal remains. The results of this study will contribute to solve the forensic cases dealing with skeletal remains. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 275~280 | - |
dc.relation.isPartOf | FORENSIC SCIENCE INTERNATIONAL-GENETICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Bone and Bones/metabolism* | - |
dc.subject.MESH | DNA/isolation & purification* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Microsatellite Repeats | - |
dc.subject.MESH | Polymerase Chain Reaction/methods | - |
dc.title | Simple and highly effective DNA extraction methods from old skeletal remains using silica columns | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Forensic Medicine (법의학) | - |
dc.contributor.googleauthor | Hwan Young Lee | - |
dc.contributor.googleauthor | Myung Jin Park | - |
dc.contributor.googleauthor | Na Young Kim | - |
dc.contributor.googleauthor | Jeong Eun Sim | - |
dc.contributor.googleauthor | Woo Ick Yang | - |
dc.contributor.googleauthor | Kyoung-Jin Shin | - |
dc.identifier.doi | 10.1016/j.fsigen.2009.10.014 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01456 | - |
dc.contributor.localId | A02085 | - |
dc.contributor.localId | A02210 | - |
dc.contributor.localId | A03335 | - |
dc.contributor.localId | A00349 | - |
dc.relation.journalcode | J00905 | - |
dc.identifier.eissn | 1878-0326 | - |
dc.identifier.pmid | 20457067 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1872497310000050 | - |
dc.subject.keyword | DNA extraction | - |
dc.subject.keyword | Skeletal remains | - |
dc.subject.keyword | Complete demineralization | - |
dc.subject.keyword | Silica-based column | - |
dc.subject.keyword | Polymerase chain reaction inhibitor | - |
dc.contributor.alternativeName | Kim, Na Young | - |
dc.contributor.alternativeName | Park, Myung Jin | - |
dc.contributor.alternativeName | Shin, Kyoung Jin | - |
dc.contributor.alternativeName | Sim, Jeong Eun | - |
dc.contributor.alternativeName | Lee, Hwan Young | - |
dc.contributor.affiliatedAuthor | Park, Myung Jin | - |
dc.contributor.affiliatedAuthor | Shin, Kyoung Jin | - |
dc.contributor.affiliatedAuthor | Sim, Jeong Eun | - |
dc.contributor.affiliatedAuthor | Lee, Hwan Young | - |
dc.contributor.affiliatedAuthor | Kim, Na Young | - |
dc.citation.volume | 4 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 275 | - |
dc.citation.endPage | 280 | - |
dc.identifier.bibliographicCitation | FORENSIC SCIENCE INTERNATIONAL-GENETICS, Vol.4(5) : 275-280, 2010 | - |
dc.identifier.rimsid | 46674 | - |
dc.type.rims | ART | - |
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