Cited 83 times in
Effect of decorin on overcoming the extracellular matrix barrier for oncolytic virotherapy.
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김호근 | - |
dc.contributor.author | 유지영 | - |
dc.contributor.author | 윤채옥 | - |
dc.contributor.author | 이영숙 | - |
dc.contributor.author | 최일규 | - |
dc.contributor.author | 김동석 | - |
dc.date.accessioned | 2015-04-23T16:25:48Z | - |
dc.date.available | 2015-04-23T16:25:48Z | - |
dc.date.issued | 2010 | - |
dc.identifier.issn | 0969-7128 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/100566 | - |
dc.description.abstract | The pressing challenge for contemporary gene therapy is to deliver enough therapeutic genes to enough cancer cells in vivo. With the aim of improving viral distribution and tumor penetration, we explored the use of decorin to enhance viral spreading and tumor tissue penetration. We generated decorin-expressing replication-incompetent (dl-LacZ-DCNG, dl-LacZ-DCNQ and dl-LacZ-DCNK) and replication-competent (Ad-DeltaE1B-DCNG, Ad-DeltaE1B-DCNQ and Ad-DeltaE1B-DCNK) adenoviruses (Ads). Point mutants of decorin gene (DCNG), DCNK and DCNQ, have a negative and moderate binding affinity to type-I collagen fibril, respectively. In both tumor spheroids and established solid tumors in vivo, tissue penetration potency of dl-LacZ-DCNG was greatly enhanced than those of dl-LacZ, dl-LacZ-DCNQ and dl-LacZ-DCNK, and this enhanced tissue penetration effect derived from decorin-expressing Ad was dependent on the binding affinity of decorin to collagen fibril. Expression of DCNG enhanced viral spread of replicating Ad, leading to improved tumor reduction and survival benefit. Moreover, the tumoricidal effects of Ad-DeltaE1B-DCNQ and Ad-DeltaE1B-DCNK were lessened, as the binding affinity to collagen was decreased, showing that the increased cancer cell cytotoxicity was driven by the action of decorin on extracellular matrix (ECM). Furthermore, Ad-DeltaE1B-DCNG substantially decreased ECM components within the tumor tissue. Finally, intratumoral injection of Ad-DeltaE1B-DCNG in primary tumor site greatly reduced the formation of B16BL6 melanoma cell pulmonary metastases in mice. Taken together, these data show the utility of decorin as a dispersion agent and highlight its utility and potential in improving the efficacy of replicating Ad-mediated cancer gene therapy. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 190~201 | - |
dc.relation.isPartOf | GENE THERAPY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenoviridae/genetics* | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Decorin | - |
dc.subject.MESH | Extracellular Matrix/metabolism* | - |
dc.subject.MESH | Extracellular Matrix Proteins/genetics* | - |
dc.subject.MESH | Extracellular Matrix Proteins/metabolism | - |
dc.subject.MESH | Gene Transfer Techniques | - |
dc.subject.MESH | Genetic Therapy | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Nude | - |
dc.subject.MESH | Oncolytic Virotherapy/methods* | - |
dc.subject.MESH | Proteoglycans/genetics* | - |
dc.subject.MESH | Proteoglycans/metabolism | - |
dc.subject.MESH | Spheroids, Cellular/metabolism | - |
dc.subject.MESH | Transduction, Genetic | - |
dc.subject.MESH | Xenograft Model Antitumor Assays | - |
dc.title | Effect of decorin on overcoming the extracellular matrix barrier for oncolytic virotherapy. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pathology (병리학) | - |
dc.contributor.googleauthor | I-K Choi | - |
dc.contributor.googleauthor | Y-S Lee | - |
dc.contributor.googleauthor | J Y Yoo | - |
dc.contributor.googleauthor | A-R Yoon | - |
dc.contributor.googleauthor | H Kim | - |
dc.contributor.googleauthor | D-S Kim | - |
dc.contributor.googleauthor | D G Seidler | - |
dc.contributor.googleauthor | J-H Kim | - |
dc.contributor.googleauthor | C-O Yun | - |
dc.identifier.doi | 10.1038/gt.2009.142 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01183 | - |
dc.contributor.localId | A02516 | - |
dc.contributor.localId | A02614 | - |
dc.contributor.localId | A02958 | - |
dc.contributor.localId | A04168 | - |
dc.contributor.localId | A00402 | - |
dc.relation.journalcode | J00924 | - |
dc.identifier.eissn | 1476-5462 | - |
dc.identifier.pmid | 19907500 | - |
dc.identifier.url | http://www.nature.com/gt/journal/v17/n2/full/gt2009142a.html | - |
dc.contributor.alternativeName | Kim, Ho Keun | - |
dc.contributor.alternativeName | Yoo, Ji Yeong | - |
dc.contributor.alternativeName | Yun, Chae Ok | - |
dc.contributor.alternativeName | Lee, Young Sook | - |
dc.contributor.alternativeName | Choi, Il Kyu | - |
dc.contributor.alternativeName | Kim, Dong Seok | - |
dc.contributor.affiliatedAuthor | Kim, Ho Keun | - |
dc.contributor.affiliatedAuthor | Yoo, Ji Yeong | - |
dc.contributor.affiliatedAuthor | Yun, Chae Ok | - |
dc.contributor.affiliatedAuthor | Lee, Young Sook | - |
dc.contributor.affiliatedAuthor | Choi, Il Kyu | - |
dc.contributor.affiliatedAuthor | Kim, Dong Seok | - |
dc.citation.volume | 17 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 190 | - |
dc.citation.endPage | 201 | - |
dc.identifier.bibliographicCitation | GENE THERAPY, Vol.17(2) : 190-201, 2010 | - |
dc.identifier.rimsid | 36571 | - |
dc.type.rims | ART | - |
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